Conference Proceedings
Reversibility of retinal ganglion cell dysfunction due to chronic IOP elevation.
Da Zhao, Vickie Hoi Ying Wong, Zheng He, Christine Tram Oanh Nguyen, Andrew Ian Jobling, Erica Fletcher, Holly Chinnery, Patricia Jusuf, Jeremiah KH Lim, Algis J Vingrys, Bang V Bui
Investigative Ophthalmology & Visual Science | Association for Research in Vision and Ophthalmology | Published : 2018
Abstract
Purpose : To determine the duration of chronic IOP elevation beyond which ganglion cell function can no longer recover using the mouse circumlimbal suture model. Methods : IOP elevation was induced in anaesthetized (isoflurane) adult male C57BL6/J mice by attaching a circumlimbal suture (nylon, 10/0) around the equator of one eye, with the contralateral eye serving as a control. The suture was left in place for 8, 12 and 16 weeks (n=27, 23 and 27), respectively, and animals underwent electroretinography and optical coherence tomography at these time points. In two other groups, the suture was removed after 8 and 12 weeks (n=26 and 28), and the capacity for recovery assessed 4 weeks later. ..
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